Toxicological properties of an aqueous extract of Aristolochia triangularis leaves, using the brine shrimp lethality and Allium cepa bioassays

Author:

Silva José Darlan Alves da1,Nogueira Cláudio Rodrigo2ORCID,Vieira Maria do Carmo2,Heredia-Vieira Silvia Cristina1,Barufatti Alexeia2ORCID,Crispim Bruno do Amaral2,Francisco Luiza Flávia Veiga2,Viana Lucilene Finoto1,Cardoso Claudia Andrea Lima1

Affiliation:

1. Universidade Estadual de Mato Grosso do Sul (UEMS), Brazil

2. Universidade Federal da Grande Dourados (UFGD), Brazil

Abstract

ABSTRACT: The aqueous extract of Aristolochia triangularis leaves showed activity against Artemia salina larvae, with an LD50 of 370.6 µg/mL. In experiments with Allium cepa seeds, the extract caused a 51.26% reduction in the germination index, inhibited mean root growth, and was cytotoxic at concentrations of 668 and 2,000 µg/mL. Its antioxidant activity was additionally assessed in this research and the ferric reducing antioxidant power value was found to be 391.2 µM/g. Such health-beneficial property can be attributed partly to the total phenolic content, spectrophotometrically determined as 52.67 mg/g. The occurrence of cytotoxicity suggested caution when consuming teas from A. triangularis leaves for medicinal purposes, and equally reveals the need for further studies to investigate their adverse effects.

Publisher

FapUNIFESP (SciELO)

Subject

General Veterinary,Agronomy and Crop Science,Animal Science and Zoology

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