Selenium supplementation prevents DNA damage in ram spermatozoa

Author:

Moya Carla Fredrichsen1ORCID,Piagentini Marcelo2ORCID,Silva Danilo da Cunha3ORCID,Fernandes Fábio Henrique4ORCID,Salvadori Daisy Maria Fávero4ORCID,Oba Eunice4ORCID

Affiliation:

1. Universidade Estadual do Centro-Oeste (UNICENTRO), Brazil

2. Faculdade Eduvale, Brazil

3. , Brasil

4. Universidade Estadual Paulista “Júlio de Mesquita Filho” (UNESP), Brazil

Abstract

ABSTRACT: In the present study, we aimed to evaluate the effects of different concentrations of selenium (Se) ovine nutritional supplementation on spermatozoa DNA integrity. Thirty male ovines (age: 10 months) were used. They were fed with hay and ram food in an intensive system, which was divided into stalls (5 m long and 3 m wide) with feeding troughs, and had ad libitum access to food and water. Ovines in group 1 (G1, the negative control) received mineral salt supplementation without Se; ovines in G2 received the same mineral salt mixed with 5 mg Se (as sodium selenite)/kg mineral supplement;ovines in G3 received 10 mg Se/kg mineral supplement; ovines in G4 received 15 mg Se/kg mineral supplement; and ovines in G5 received 20 mg Se/kg mineral supplement. Ovines in all groups remained untreated for 14 days, followed by a treatment period of 56 days. Semen samples were obtained by electroejaculation. The DNA damage in semen samples was evaluated using the comet assay. The experimental design was implemented using a 5 × 5 Latin Square, i.e., five treatments and five experimental periods. The mean differences were compared using Tukey’s test at a significance level of 5%. The control group (G1) showed a high percentage of DNA damage compared to the Se-treated groups (G2-G5). Therefore, Se supplementation could decrease the basal level of DNA damage in sperm cells, suggesting that Se might exert protective effects on sperm DNA.

Publisher

FapUNIFESP (SciELO)

Subject

General Veterinary,Agronomy and Crop Science,Animal Science and Zoology

Reference29 articles.

1. Sperm DNA damage assessment: a test whose time has come;AGARWAL A.;Fertility and Sterility,2005

2. Selenium and endocrine systems;BECKETT G. J.;Journal of Endocrinology,2005

3. Effects of selenium deficiency on testicular morphology and function in rats.;BEHNE D.;Journal of Reproductive and Fertility,1996

4. The ultra-structure of some characteristic sperm defects and a proposal for a new classification of the Bull spermiogram.;BLOM E.;Nordisk Veterinarer Medicin,1973

5. Selenium, a key element in spermatogenesis and male fertility;BOITANI C.;Advances in experimental medicine and biology,2009

Cited by 2 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3