Stability in chromosome number and DNA content in synthetic tetraploids of Lolium multiflorum after two generations of selection

Author:

Pereira Roselaine Cristina1,Santos Natália de Souza1,Bustamante Fernanda de Oliveira1,Mittelmann Andrea2,Techio Vânia Helena1

Affiliation:

1. Universidade Federal de Lavras, Brazil

2. Embrapa Clima Temperado, Brazil

Abstract

ABSTRACT: Chromosome doubling of Italian ryegrass genotypes ( Lolium multiflorum Lam.) adapted to the brazilian edaphoclimatic conditions is an important strategy used by breeders and aims to obtain more vigorous genotypes with better forage quality and disease resistance. The effectiveness of chromosome doubling can be measured by genetic stability and fertility rates of plants over generations. However, a common problem in the polyploidization process is the regeneration of mixoploid plants that have impaired fertility and genetic stability. The objective of this study was to verify if progenies of recently tetraploidized plants remain stable regarding DNA content and chromosome number, over two generations. Progenies of L. multiflorum plants artificially tetraploidized with colchicine treatment were evaluated. Chromosome counting and estimates of the DNA content were used to evaluate the genetic stability. The percentage of tetraploid plants (4X) increased over generations (18%, 34% and 91% in cycle 0, 1 and 2, respectively). All progenies identified as tetraploid by flow citometry showed variation in chromosome number (mixoploidy), but produced viable seeds. Results showed that stabilization in chromosome number and DNA content in tetraploidized plant progenies requires time and that the success of this procedure depends on a continuous and accurate screening and selection.

Publisher

FapUNIFESP (SciELO)

Subject

General Veterinary,Agronomy and Crop Science,Animal Science and Zoology

Reference14 articles.

1. Pesquisa Agropecuária Brasileira;ABREU J.C. de.,2006

2. Chromosome duplication of triploid hybrids between elephantgrass and pearl millet.;BARBOSA S.;Bragantia,2007

3. In vitro induction of hexaploid plants from triploid hybrids of Pennisetum purpureum and Pennisetum glaucum.;CAMPOS J.M.S.;Plant Breeding,2009

4. Changes in allele frequencies in colchicine-treated ryegrass populations assessed with RAPD markers.;CASTRO C.M.;Revista Brasileira de Agrociência,2003

5. Application of flow cytometry for the study of plants genomes;DOLEZEL J.;Journal of Applied Genetics,1997

Cited by 13 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3