Somatic cell count obtained by Ekomilk Scan® and correlations with other methods of analysis

Author:

Gonçalves Ana Carolina Siqueira1ORCID,Roma Júnior Luiz Carlos2ORCID,Privatti Rafaela Torrezan3ORCID,Salles Márcia Saladini Vieira2ORCID,Paz Claudia Cristina Paro de2ORCID,Zadra Lenira El Faro2ORCID,Vidal Ana Maria Centola4ORCID

Affiliation:

1. Universidade Estadual Paulista “Júlio de Mesquita Filho”, Brazil

2. Instituto de Zootecnia, Brasil

3. Universidade de São Paul, Brazil

4. Universidade de São Paulo, Brazil

Abstract

ABSTRACT: The study aimed to evaluate the equipment Ekomilk Scan® as an alternative to somatic cell count (SCC) in milk. For this individual cow milk samples of various ages and different stages of lactation in northeastern state of São Paulo region were collected. The analyzes performed were divided into variables related to the equipment: repeatability and reproducibility, and variables that could influencing the results as: use of preservatives, temperature, time between collection and analysis, breed and milk composition, besides analysis to relate the Ekomilk Scan® with the standard method-direct microscopy and reference-flow cytometry. As the result, for samples analysis, it shouldn’t be added preservative and these should be conducted on the same day of collection; however, temperature sample did not significantly influence results. Furthermore, Ekomilk Scan® did not show good correlation of results with the method of direct microscopy; however, it was necessary to generate equations for a positive correlation between flow cytometry and Ekomilk Scan®. Therefore, it is concluded that the equipment tested is not accurate but it can be an alternative for SCC monitoring in productive units since it uses calibration equations of results.

Publisher

FapUNIFESP (SciELO)

Subject

General Veterinary,Agronomy and Crop Science,Animal Science and Zoology

Reference23 articles.

1. Avaliação de testes rápidos para análise da contagem de células somáticas de leite cru de tanques;ARAÚJO V.M.;Acta Veterinaria Brasilica,2012

2. Influence of shipping container, preservative and breed on analysis of milk components of shipped samples.;BERTRAND J.A.;Journal of Dairy Science,1996

3. Instrução Normativa nº 62. Secretaria de Defesa Agropecuária, Ministério da Agricultura Pecuária e Abastecimento;Diário Oficial da União,2011

4. Validação de métodos analíticos: estratégia e discussão;BRITO N.M.,2003

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