Histopathological, immunohistochemical and biomolecular diagnosis of myocarditis due to Clostridium chauvoei in a bovine

Author:

Casagrande Renata Assis1,Pires Prhiscylla Sadanã2,Silva Rodrigo Otávio Silveira2,Sonne Luciana3,Borges João Batista Souza3,Neves Monique Silva2,Rolim Veronica Machado3,Souza Suyene Oltramari de3,Driemeier David3,Lobato Francisco Carlos Faria2

Affiliation:

1. Universidade do Estado de Santa Catarina, Brazil

2. Universidade Federal de Minas Gerais, Brazil

3. Universidade Federal do Rio Grande do Sul, Brazil

Abstract

The aim of this study was to report a case of clostridial myocarditis in a bovine in Brazil with emphasis on the pathological findings, isolation and molecular identification associated with the in situ localization of C. chauvoei. The animal, a male Brangus bull with nine months of age, was found dead without prior clinical signs. Multifocal and coalescent areas of necrosis were observed in the myocardium. Rod cells in the cardiac muscle fibers were positive immunostaining for C. chauvoei, while this bacterium was also isolated and identified by polymerase chain reaction (PCR).

Publisher

FapUNIFESP (SciELO)

Reference13 articles.

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2. Cytotoxin CctA, a major virulence factor of Clostridium chauvoei conferring protective immunity against myonecrosis.;FREY J.;Vaccine,2012

3. Histotoxic clostridia.;GYLES C.L.;Clostridia,1993

4. Novel real-time PCR assay for simultaneous detection and differentiation of Clostridium chauvoei and Clostridium septicum in clostridial myonecrosis.;HALM A.;Journal of Clinical of Microbiology,,2010

5. Outbreak of intestinal and lingual Clostridium chauvoei infection in two-year-old Friesian heifers.;HARWOOD D.G.;Veterinary Record,2007

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