Affiliation:
1. Centro de Pesquisa Leônidas e Maria Deane Fiocruz-Amazônia, Brasil; London School of Hygiene and Tropical Medicine, United Kingdom
2. Instituto Oswaldo Cruz, Brasil
Abstract
Chagas disease control initiatives are yielding promising results. Molecular research has helped successful programs by identifying and characterizing introduced vector populations and by defining intervention targets accurately. However, researchers and health officials are facing new challenges throughout Latin America. Native vectors persistently reinfest insecticide-treated households, and sylvatic triatomines maintain disease transmission in humid forest regions (including Amazonia) without colonizing human dwellings. In these scenarios, fine-scale vector studies are essential to define epidemiological risk patterns and clarify the involvement of little-known triatomine taxa in disease transmission. These eco-epidemiological investigations, as well as the planning and monitoring of control interventions, rely by necessity on accurate taxonomic judgments. The problems of cryptic speciation and phenotypic plasticity illustrate this need - and how molecular systematics can provide the fitting answers. Molecular data analyses also illuminate basic aspects of vector evolution and adaptive trends. Here we review the applications of molecular markers (concentrating on allozymes and DNA sequencing) to the study of triatomines. We analyze the suitability, strengths and weaknesses of the various techniques for taxonomic, systematic and evolutionary investigations at different levels (populations, species, and higher taxonomic categories).
Reference102 articles.
1. The ecology and genetics of Chagas disease vectors in Ecuador, with emphasis on Rhodnius ecuadoriensis (Triatominae);ABAD-FRANCH F,2003
2. Phylogenetic relationships among members of the Pacific Rhodnius lineage (Hemiptera: Reduviidae: Triatominae);ABAD-FRANCH F;Infect Genet Evol,2003
3. Identification and characterization of microsatellite markers in the Chagas disease vector Triatoma dimidiata;ANDERSON JM;Infect Genet Evol,2002
4. Molecular markers, natural history and evolution;AVISE JC,1994
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