Affiliation:
1. University of São Paulo, Brazil
2. Federal University of São Carlos, Brazil
Abstract
The present study evaluated the progression of osteogenic cell cultures exposed to a novel calcium aluminate cement (CAC+) in comparison with the gold standard mineral trioxide aggregate (MTA). Cells were enzimatically isolated from newborn rat calvarial bone, plated on glass coverslips containing either CAC+ or a control MTA samples in the center, and grown under standard osteogenic conditions. Over the 10-day culture period, roundening of sample edges was clearly noticed only for MTA group. Although both cements supported osteogenic cell adhesion, spreading, and proliferation, CAC+-exposed cultures showed significantly higher values in terms of total cell number at days 3 and 7, and total protein content and alkaline phosphatase activity at day 10. The present in vitro results indicate that the exposure to CAC+ supports a higher differentiation of osteogenic cells compared with the ones exposed to MTA. Further experimental studies should consider CAC+ as a potential alternative to MTA when the repair of mineralized tissues is one of the desired outcomes in endodontic therapy.
Reference20 articles.
1. Cellular response to Mineral Trioxide Aggregate;Koh ET;J Endod,1998
2. Osteoblast biocompatibility of mineral trioxide aggregate;Mitchell PJ;Biomaterials,1999
3. Adhesion of human osteoblasts on root-end filling materials;Zhu Q;J Endod,2000
4. Human alveolar bone cells interact with ProRoot and tooth-colored MTA;Al-Rabeah E;J Endod,2006
5. A composition based on aluminate cement for application in endodontics and the obtained cement product. Patent registration INPI 0704502-6;Pandolfelli VC,2007
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