Affiliation:
1. University of São Paulo, Brazil
2. Fukuoka University, Japan
3. Federal University of São Paulo, Brazil
Abstract
Togue mucosa surface of 3-day postnatal rats was examined under transmission electron microscopy (TEM) and high-resolution scanning electron microscopy (HRSEM). For HRSEM analysis, the specimens were fixed in the same solution for 24 h, postfixed in 2% osmiun tetroxide, critical-point dried and coated with platinum-palladium. For TEM analysis, the specimens were fixed using modified Karnovsky solution and embedded in Spurr resin. The results revealed the presence of numerous microplicae in the membrane surface of keratinized epithelial cells to which groups of bacteria were attached. These bacteria were staphylococcus and coccus organized either in rows or at random, which were visualized in three-dimensional HRSEM images. At high magnification, the TEM images revealed the adhesion of bacteria to the cell membrane through numerous filamentous structures comprising the glycocalyx. The fine fibrillar structures rising from each bacterium and from cell membrane were clearly seen. These characteristics on bacteria structure may be used for future control or prevention of bacterial diseases and for installation of the oral native flora.
Reference17 articles.
1. Surface structure of tongue, palate and buccal mucosa of rat by scanning electron microscopic study;Yoshioka I;Okaj Folia Anat,1976
2. Three-dimensional ultrastructure of the surface of the tongue of the rat snake: Elaphe climacophora;Iwasaki S;Anat Rec,1996
3. Fine structure of the dorsal lingual epithelium of the juvenile hawksbill turtle: Gretmochelys imbriata bissa;Iwasaki S;Anat Rec,1996
4. Three-dimensional architecture of the connective tissue core and surface structures of the lingual papillae in the rabbit;Silva MC;Histol Histopathol,2002
5. Adherence of bacteria to oral epithelium in vivo: electron microscopic observations;Barnett ML;J Dent Res,1973
Cited by
3 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献