Optimizing Fixation Protocols to Improve Molecular Analysis from FFPE Tissues-Reference-Cited by-同舟云学术

Optimizing Fixation Protocols to Improve Molecular Analysis from FFPE Tissues

Published:2017-02 Issue:1 Volume:28 Page:82-84
ISSN:0103-6440
Container-title:Brazilian Dental Journal
language:
Short-container-title:Braz. Dent. J.

Author:

Maraschin Bruna Jalfim¹,Silva Viviane Palmeira da¹,Rock Leigha²,Sun Huichen³,Visioli Fernanda¹,Rados Pantelis Varvaki¹,Rosin Miriam P.³

Affiliation:

1. Universidade Federal do Rio Grande do Sul, Brazil

2. University of British Columbia, Canada

3. British Columbia Cancer Agency Research Centre, Canada

Abstract

Abstract Most Departments of Pathology around the world have a considerable archive of formalin-fixed paraffin-embedded (FFPE) tissue suitable for molecular assessment. This article points out the potential DNA damage that may occur if basic steps are not followed during processing and storage of these samples. Furthermore, it hopes to establish parameters to optimize quality and quantity of DNA extracted from FFPE tissues.

Publisher

FapUNIFESP (SciELO)

Subject

General Dentistry

Link

http://www.scielo.br/pdf/bdj/v28n1/1806-4760-bdj-28-01-00082.pdf

Reference13 articles.

1. Unlocking the molecular archive: the emerging use of formalin-fixed paraffin-embedded tissue for biomarker research in urological cancer;Gnanapragasam VJ;BJU Int,2010

2. The storage period of the formalin-fixed paraffin-embedded tumor blocks does not influence the concentration and purity of the isolated DNA in a series of 83 renal and thyroid carcinomas;Nechifor-Boilă AC;Rom J Morphol Embryol,2015

3. Molecular analysis of oral lichen planus. A premalignant lesion?;Zhang L;Am J Pathol,1997

4. Effect of fixatives and tissue processing on the content and integrity of nucleic acids;Srinivasan M;Am J Pathol,2002

5. DNA damage in preserved specimens and tissue samples: a molecular assessment;Zimmermann J;Front Zool

Cited by 4 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. INSTAPRESERVETM: AN ALTERNATIVE FIXATIVE TO FORMALIN AND SUITABLE FOR HISTOPATHOLOGY;International Journal of Pharmacy and Pharmaceutical Sciences;2024-08-01

2. Towards molecular autopsies: Development of a FFPE tissue DNA extraction workflow;Science & Justice;2022-03

3. Nested PCR for the Diagnosis of Feline Sporotrichosis From Formalin-Fixed and Paraffin-Embedded Samples Using Different DNA Extraction Protocols;Frontiers in Veterinary Science;2022-01-05

4. Alternative tissue fixation for combined histopathological and molecular analysis in a clinically representative setting;Histochemistry and Cell Biology;2021-12-14