Analysis of Molecular Changes Induced By Mineral Trioxide Aggregate On sPLA2-Reference-Cited by-同舟云学术

Analysis of Molecular Changes Induced By Mineral Trioxide Aggregate On sPLA2

Published:2019-10 Issue:5 Volume:30 Page:453-458
ISSN:1806-4760
Container-title:Brazilian Dental Journal
language:
Short-container-title:Braz. Dent. J.

Author:

Lopes Murilo B¹^ORCID,Soares Veronica C.G²^ORCID,Fagundes Fabio H.R³^ORCID,Gonini-Junior Alcides¹^ORCID,Kaneshima Renan H¹^ORCID,Guiraldo Ricardo D¹^ORCID,Diz-Filho Eduardo B.S.⁴^ORCID,Berger Sandrine B¹^ORCID,Felizardo Klissia R⁵^ORCID,Santos Marcelo L. dos⁶^ORCID

Affiliation:

1. Universidade Norte do Paraná, Brazil

2. Universidade Paulista, Brazil

3. Universidade Padre Anchieta, Brazil

4. Universidade Estadual de Campinas, Brazil

5. Universidade Paranaense, Brazil

6. Universidade Federal de Sergipe, Brazil

Abstract

Abstract The aim of this study was to analyze the effects of MTA on the structure and enzymatic activity of sPLA2 in order to provide subsidies for improvement in the formulation of the product. MTA powder was incubated for 60 min in the presence of sPLA2 and was analyzed by chromatography, electrospray mass (ESI-MS) and small-angle X-ray scattering (SAXS). It was find that the elution profile, retention time, and fragmentation of sPLA2 were altered after treatment with MTA. Calcium was the MTA component that most amplified the inflammatory signal. Significant interactions were found between MTA and sPLA2, which could aid in our understanding of the mechanisms of action of MTA during the inflammatory process and it may facilitate the structural modification of MTA, thereby improving its biological safety and consequently the rate of the treatment success.

Publisher

FapUNIFESP (SciELO)

Subject

General Dentistry

Link

http://www.scielo.br/pdf/bdj/v30n5/1806-4760-bdj-30-05-453.pdf

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