Fluoride uptake in dental enamel after using fluoridated dentifrice, preceded or not by a CaCl2 solution rinse

Abstract

INTRODUCTION: The use of a calcium solution previously to brushing could favor the remineralization and the fluoride (F) uptake in dental enamel. OBJECTIVE: This study evaluated the F in dental enamel after using a F dentifrice, preceded or not by a rinse with a CaCl2 solution. METHODS: Ten subjects (18-30 years) participated in a double-blind, cross-over protocol. Enamel biopsy and 3 min saliva samples were obtained at baseline and after brushing (1.5 g Crest®, 1,000 ppm F as NaF), preceded by a rinse with 10 mL of 20 mM CaCl2 50 mM acetate pH 7.2 or deionized water, for 1 min, followed by a 15 mL water rinse for 5 sec. Biopsies were made before the rinses and after 8-15 and 120 min. Saliva samples were collected before the rinses and after 4, 15, 30, 60 and 120 min. F concentration in enamel biopsies was analyzed with the F electrode (Orion 9409) and a miniature calomel reference electrode, while phosphorus concentration was analyzed by spectrophotometry. Saliva samples were analyzed for F with the electrode (Orion 9609) by the direct method. The data were analyzed by ANOVA and Tukey test (p<0.05). RESULTS: Mean F concentration ± SD (µg/mL) at baseline up to 120 min for saliva samples were: 0.02±0.01; 9.06±4.26; 2.01±1.76; 0.47±0.33; 0.24±0.17; 0.08±0.08; 0.07±0.04 for the deionized water rinse, and 0.02±0.02; 10.96±14.21; 2.76±3.04; 0.87±1.29; 0.40±0.66; 0.16±0.23; 0.09±0.09 for the CaCl2 rinse. The data for enamel biopsies were (µg/g): 1861.7±1011.0; 1790.5±953.2; 1117.6±585.2 for the deionized water rinse, and 1586.4±776.8; 1536.3±546.4; 1520.5±1340.7 for the CaCl2 rinse. CONCLUSION: According to this protocol, there was not a significant variation in enamel F uptake after using a F dentifrice preceded or not by a rinse with a CaCl2 solution.