Author:
Galvão Letícia Oba,Moreira Júnior Sebastião,Medeiros Júnior Pedro,Lemos Gleiser José Piantino,Cunha Nara Fabiana,Antonino Rosa Maria Parreiras,Santos Filho Bráulio Silva,Magalhães Albino Verçosa
Abstract
One hundred and eighty-two male inbred C57/BL/6 mice were infected with 3 x 106 Leishmania (Leishmania) amazonensis promastigotes of the MHOM/BR/PH8 strain by means of a subcutaneous injection in the right ear. The animals were separated in three groups: 1) oral mefloquine hydrochloride treatment (16mg/kg/day/10 days), 2) intramuscular aminosidine (Paromomycin®) treatment (20mg/kg/20 days) and 3) control. Twenty six mice of each treated group were sacrificed, one at the end of treatment (nine weeks after inoculation), and one six weeks later (fifteen weeks after inoculation). Control Group animals were sacrificed at weeks six, nine and fifteen after inoculation. There was no significant difference between Group 1 (mefloquine) and Group 3 (control) subjects. Group 2 animals (aminosidine) presented the smallest differences of all, both at the end of the treatment and six weeks later. The histopato-logical parameters have shown the following findings: a) there was no significant difference between the mefloquine treated group and the control group; the group treated with aminosidine showed fewer of vacuolated macrophages than the control group, at week 9 (end of treatment). b) both at the end of treatment and six weeks later, evaluation of tissue necrosis and tissue fibrosis revealed no differences between the treated groups. It was found that six weeks after the end of treatment, mice in the control group presented significantly more severe degrees of fibrosis than mice in the other groups. It can be concluded that mefloquine showed limited therapeutic effect in this experimental model, whereas aminosidine had a significant effect. Nevertheless, neither of them resulted in cure of the lesions.
Subject
Infectious Diseases,Microbiology (medical),Parasitology
Reference22 articles.
1. Electrocardiographic alterations during treatment of mucocutaneous leishmaniasis with antimoniate and allopurinol;Antezana G;Transactions of the Royal Society of Tropical Medicine and Hygiene,1992
2. Chemotherapy for Leishmaniasis: biochemical mechanisms, clinical efficacy, and future strategies;Berman JD;Reviews of Infectious Diseases,1988
3. Aminosidine (paromomycin) in the treatment of leishmaniasis imported into the United Kingdom;Bryceson ADM;Transactions of the Royal Society of Tropical Medicine and Hygiene,1992
4. Severe arthralgia, not related to doses, associated with pentavalent antimonial therapy for mucosal leishmaniasis;Castro C;Transactions of the Royal Society of Tropical Medicine and Hygiene,1990
5. Leishmaniasis: recent developments in chemotherapy;Cook CG;Journal of Antimicrobial Chemotherapy,1993
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