Low sensitivity of polymerase chain reaction for diagnosis of tuberculous meningitis in southeastern Brazil

Author:

Brienze Vânia Maria Sabadoto1,Tonon Ângela Pedroso1,Pereira Fabrício José Tarelho1,Liso Elisabete1,Tognola Waldir Antônio1,Santos Manoel Armando Azevedo dos2,Ferreira Marcelo Urbano2

Affiliation:

1. Faculdade de Medicina de São José do Rio Preto

2. Universidade de São Paulo

Abstract

Two polymerase chain reaction (PCR) protocols showed low sensitivity (36% and 53% for TB AMPLICOR and MPB64 nested PCR, respectively), when compared with classic microbiological methods (73% and 54% for Ziehl-Neelsen staining and culture, respectively), in the diagnosis of tuberculous meningitis in 91 patients in southeastern Brazil. Only three PCR-positive, microbiologically negative patients were found. Analysis of sequential cerebrospinal fluid samples by nested PCR detected Mycobacterium tuberculosis DNA up to 29 days after the introduction of antituberculosis chemotherapy.

Publisher

FapUNIFESP (SciELO)

Subject

Infectious Diseases,Microbiology (medical),Parasitology

Reference21 articles.

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3. Diagnostic standards and classification of tuberculosis;American Review of Respiratory Diseases,1990

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