Purification and characterization of hyaluronic acid from chicken combs-Reference-Cited by-同舟云学术

Purification and characterization of hyaluronic acid from chicken combs

Published:2012-07-19 Issue:9 Volume:42 Page:1682-1687
ISSN:1678-4596
Container-title:Ciência Rural
language:
Short-container-title:Cienc. Rural

Author:

Rosa Claudia Severo da¹,Tovar Ana Freire²,Mourão Paulo²,Pereira Ricardo²,Barreto Pedro³,Beirão Luiz Henrique³

Affiliation:

1. Universidade Federal de Santa Maria (UFSM), Brasil

2. Universidade Federal do Rio de Janeiro (UFRJ), Brasil

3. Universidade Federal de Santa Catarina (UFSC), Brasil

Abstract

Hyaluronic acid (HA) is an important macromolecule in medical and pharmaceutical fields. The umbilical cord and the chicken comb are some of the tissues richest in this polysaccharide. The profit from obtaining HA from the combs of slaughtered animals is particularly attractive. This work aimed to extract, purify, and characterize HA. The glycosaminoglycan concentration in the chicken comb was found to be about 15µg of hexuronic acid mg-1 of dry tissue. Fractionation using ion exchange chromatography and subsequent identification of the fractions by agarose gel electrophoresis showed that HA corresponded to 90% of the total amount of extracted glycosaminoglycans.

Publisher

FapUNIFESP (SciELO)

Link

http://www.scielo.br/pdf/cr/v42n9/a24712cr4426.pdf

Reference29 articles.

1. Implantable therapy system and methods;AEBISCHER P.,2001

2. Hyaluronan;ALMOND A.;Cellular Mol Life Science,2007

3. C14 Assays and autoradiografic studies on the rooster comb;BALAZS E.A.;Journal Biophysica and Biochemistry Cytology,1958

4. Hyaluronic acid containing hydrogels for the reduction of protein adsorption;BEEK M.;Biomaterials,2008

5. The 13C-NMR spectra of hyaluronate and chondroitin sulphates: Further evidence on an alkali-induced conformation change;BOCIEK S.M.;Europeu Journal Biochemistry,1980

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