Expression of NMDA receptor and microRNA-219 in rats submitted to cerebral ischemia associated with alcoholism

Author:

Silva Cristiane Iozzi1,Novais Paulo Cézar2,Rodrigues Andressa Romualdo1,Carvalho Camila A.M.3,Colli Benedicto Oscar1,Carlotti Jr. Carlos Gilberto1,Tirapelli Luís Fernando1,Tirapelli Daniela P.C.1

Affiliation:

1. Universidade de São Paulo, Brasil

2. Universidade de Marília, Brasil

3. Universidade Federal de Alagoas, Brasil

Abstract

ABSTRACT Alcohol consumption aggravates injuries caused by ischemia. Many molecular mechanisms are involved in the pathophysiology of cerebral ischemia, including neurotransmitter expression, which is regulated by microRNAs. Objective: To evaluate the microRNA-219 and NMDA expression in brain tissue and blood of animals subjected to cerebral ischemia associated with alcoholism. Methods: Fifty Wistar rats were divided into groups: control, sham, ischemic, alcoholic, and ischemic plus alcoholic. The expression of microRNA-219 and NMDA were analyzed by real-time PCR. Results: When compared to the control group, the microRNA-219 in brain tissue was less expressed in the ischemic, alcoholic, and ischemic plus alcoholic groups. In the blood, this microRNA had lower expression in alcoholic and ischemic plus alcoholic groups. In the brain tissue the NMDA gene expression was greater in the ischemic, alcoholic, and ischemic plus alcoholic groups. Conclusion: A possible modulation of NMDA by microRNA-219 was observed with an inverse correlation between them.

Publisher

FapUNIFESP (SciELO)

Subject

Neurology,Clinical Neurology

Reference23 articles.

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