Affiliation:
1. Universidade Vale do Rio Doce
2. Universidade Federal de Minas Gerais
Abstract
The development of a specific agar diffusion bioassay for the quantitative determination of fluconazole formulated in capsules was carried out using a strain of Candida albicans ATCC 18804 as the test organism. A prospective validation of the method showed adequate linearity (r²=0.9995), precision (R.S.D. = 4.0% for intra-day and 4.5% for inter-day precision) and accuracy (mean recovery = 102.9%). High performance liquid chromatography was chosen as a comparison method for the fluconazole determination. The contents of fluconazole determined by both methods, for four capsule samples, showed a strong correlation, confirmed by Pearson's correlation coefficient value (r = 0.9884). The bioassay is a suitable method for both research and pharmaceutical industry laboratories.
Subject
General Pharmacology, Toxicology and Pharmaceutics
Reference31 articles.
1. Chromatographic determination of clotrimazole, ketoconazole and fluconazole in pharmaceutical formulations;ABDEL-MOETY E. M.;Farmaco,2002
2. Comparison of microbiological and UV-spectrophotometric assays for determination of voriconazole in tablets;ADAMS A. I. H.;J. AOAC Int.,1996
3. M27A2: Reference method for broth dilution antifungal susceptibility testing of yeasts: Approved standard,2002
4. Reference method for broth dilution antifungal susceptibility testing of filamentous fungi,2002
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