Affiliation:
1. University of São Paulo, Brazil
Abstract
A simple, rapid, economical and reliable high performance liquid chromatographic method has been developed and successfully applied in simultaneous determination of ethinyl estradiol and drospirenone in coated tablets. The HPLC method was performed on a LiChroCART® 100RP column (125x4 mm i.d., 5 µm) with acetonitrile:water 50:50 (v/v) as mobile phase, pumped at a flow rate of 1.0 mL.min-1. The fluorescence detection for ethinyl estradiol was made at λex= 280 nm and λem= 310 nm and a UV detection for drospirenone was made at 200 nm. The elution time for ethinyl estradiol and drospirenone were 4.0 and 5.7 min, respectively. The method was validated in accordance to USP 34 guidelines. The proposed HPLC method presented advantages over reported methods and is suitable for quality control assays of ethinyl estradiol and drospirenone in coated tablets.
Subject
General Pharmacology, Toxicology and Pharmaceutics
Reference14 articles.
1. Estudo da eficiência do tratamento de efluentes domésticos da cidade de Araraquara na remoção de hormônios sexuais;ARAÚJO J.C.,2006
2. Official methods of analysis of AOAC International,2007
3. Efficacy of a new oral contraceptive containing drospirenone and ethinyl estradiol in the long-term treatment of hirsutism;BATUKAN C.;Fertil. Steril.,2006
4. Aspectos farmacológicos e clínicos de um novo contraceptivo oral contendo drospirenona como componente progestinico;BAZZOTE R.B.;Arq. Cienc. Saúde UNIPAR,2005
5. A 1-year pharmacokinetic investigation of a novel oral contraceptive containing drospirenone in healthy female volunteers;BLODE H.;Eur. J. Contracept. Reprod. Health Care.,2000
Cited by
9 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献