Pythium insidiosum: morphological and molecular identification of Brazilian isolates

Author:

Azevedo Maria Isabel de1,Pereira Daniela I.B.2,Botton Sônia A.3,Costa Mateus M. da4,Mahl Camila D.1,Alves Sydney H.1,Santurio Janio M.1

Affiliation:

1. Universidade Federal de Santa Maria, Brazil

2. Universidade Federal de Pelotas, Brazil

3. UFSM

4. Universidade Federal do Vale do São Francisco, Brazil

Abstract

Pythium insidiosum is an oomycete belonging to the kingdom Stramenipila and it is the etiologic agent of pythiosis. Pythiosis is a life-threatening infectious disease characterized by the development of chronic lesions on cutaneous and subcutaneous, intestinal, and bone tissues in humans and many species of animals. The identification of P. insidiosum is important in order to implement a rapid and definitive diagnosis and an effective treatment. This study reports the identification of 54 isolates of P. insidiosum of horses, dogs and sheep that presented suspicious clinical lesions of pythiosis from different regions in Brazil, by using morphological and molecular assays. Throughout the PCR it was possible to confirm the identity of all Brazilian isolates as being P. insidiosum.

Publisher

FapUNIFESP (SciELO)

Subject

General Veterinary

Reference19 articles.

1. Introductory Mycology;Alexopoulos C.J.,1996

2. Gastrointestinal pythiosis in 10 dogs from Califórnia;Berryessa N.A.;J. Vet. Int. Med.,2008

3. Morphological and molecular characterization of an equine isolate of Pythium insidiosum and comparison with the first human isolate from the same geographic region;Bosco S.M.G.;Med. Mycol.,2008

4. Development of a nested polymerase chain reaction assay for the detection and identification of Pythium insidiosum;Grooters A.M.;J. Vet. Intern. Med.,2002

5. 5 S ribosomal RNA gene spacer as species specific probes for eight species of Pythium;Klassen G.R.;Phytopathol,1996

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