Crystallographic studies of fish hemoglobins

Author:

DELATORRE Plínio1,DELAMANO Márcia1,FADEL Valmir1,HONDA Rubens T.1,SMARRA André Luís S.2,CANDURI Fernanda1,OLIVIERI Johnny Rizzieri1,BONILLA-RODRIGUEZ Gustavo Orlando1,AZEVEDO Jr Walter Filgueira de1

Affiliation:

1. Universidade Estadual Paulista, Brazil

2. Centro Universitário Moacyr Sreder Bastos, Brazil

Abstract

The present work reports our succesfull experience concerning crystallization of four fish hemoglobins from three Brazilian species of Teleosts: Liposarcus anisitsi, Brycon cephalus and Piaractus mesopotamicus. The data shown here is part of a systematic functional and structural study of fish hemoglobins with the aim of better understanding the outstanding range of functional and structural properties exhibited by these proteins. We also present a reduced sparse-matrix method for crystallization of fish hemoglobins, which can reduce the amount of hemoglobin initially used in the crystallization experiments.

Publisher

FapUNIFESP (SciELO)

Subject

General Physics and Astronomy,General Chemistry

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