MULTIPLEX SYBR® GREEN-REAL TIME PCR (qPCR) ASSAY FOR THE DETECTION AND DIFFERENTIATION OF Bartonella henselae AND Bartonella clarridgeiae IN CATS-Reference-Cited by-同舟云学术

MULTIPLEX SYBR® GREEN-REAL TIME PCR (qPCR) ASSAY FOR THE DETECTION AND DIFFERENTIATION OF Bartonella henselae AND Bartonella clarridgeiae IN CATS

Published:2014-04 Issue:2 Volume:56 Page:93-95
ISSN:0036-4665
Container-title:Revista do Instituto de Medicina Tropical de São Paulo
language:
Short-container-title:Rev. Inst. Med. trop. S. Paulo

Author:

Staggemeier Rodrigo¹,Pilger Diogo André²,Spilki Fernando Rosado¹,Cantarelli Vlademir Vicente³

Affiliation:

1. Universidade Feevale, Brazil

2. Universidade Federal do Rio Grande do Sul, Brazil

3. Universidade Feevale, Brazil; Laboratório Qualitá, Brazil

Abstract

A novel SYBR® green-real time polymerase chain reaction (qPCR) was developed to detect two Bartonellaspecies, B. henselae and B. clarridgeiae, directly from blood samples. The test was used in blood samples obtained from cats living in animal shelters in Southern Brazil. Results were compared with those obtained by conventional PCR targeting Bartonella spp. Among the 47 samples analyzed, eight were positive using the conventional PCR and 12 were positive using qPCR. Importantly, the new qPCR detected the presence of both B. henselae and B. clarridgeiae in two samples. The results show that the qPCR described here may be a reliable tool for the screening and differentiation of two important Bartonella species.

Publisher

FapUNIFESP (SciELO)

Subject

Infectious Diseases,General Medicine

Link

http://www.scielo.br/pdf/rimtsp/v56n2/0036-4665-rimtsp-56-02-93.pdf

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