Standardization of dot-ELISA for the serological diagnosis of toxocariasis and comparision of the assay with ELISA-Reference-Cited by-同舟云学术

Standardization of dot-ELISA for the serological diagnosis of toxocariasis and comparision of the assay with ELISA

Published:1992-02 Issue:1 Volume:34 Page:55-60
ISSN:0036-4665
Container-title:Revista do Instituto de Medicina Tropical de São Paulo
language:
Short-container-title:Rev. Inst. Med. trop. S. Paulo

Author:

Camargo Eide Dias¹,Nakamura Paulo Mutuko¹,Vaz Adelaide José¹,Silva Marcos Vinícius da²,Chieffi Pedro Paulo³,Melo Elisabete Ourique de³

Affiliation:

1. Instituto Adolfo Lutz, Brasil

2. Instituto Adolfo Lutz, Brasil; Emilio Ribas Hospital, Brasil

3. Instituto de Medicina Tropical, Brasil

Abstract

The dot-enzyme-linked immunosorbent assay (dot-ELISA) was standardized using somatic (S) and excretory-secretory (ES) antigens of Toxocara-canis for the detection of specific antibodies in 22 serum samples from children aged 1 to 15 years, with clinical signs of toxocariasis. Fourteen serum samples from apparently normal individuals and 28 sera from patients with other pathologies were used as controls. All samples were used before and after absorption with Ascaris suum extract. When the results were evaluated in comparison with ELISA, the two tests were found to have similar sensitivity, but dot-ELISA was found to be more specific in the presence of the two antigens studied. Dot-ELISA proved to be effective for the diagnosis of human toxocariasis, presenting advantages in terms of yield, stability, time and ease of execution and low cost.

Publisher

FapUNIFESP (SciELO)

Subject

Infectious Diseases,General Medicine

Link

http://www.scielo.br/pdf/rimtsp/v34n1/a10v34n1.pdf

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