Evaluation of the human immunodeficiency virus type 1 and 2 antibodies detection in dried whole blood spots (dbs) samples

Author:

Castro Andréa Cauduro de1,Borges Luiz Gustavo dos Anjos2,Souza Ricardo da Silva de2,Grudzinski Melina3,D'Azevedo Pedro Alves3

Affiliation:

1. Fundação Faculdade Federal de Ciências Médicas de Porto Alegre, Brasil; Hospital Nossa Senhora da Conceição, Brasil

2. Universidade de Caxias do Sul, Brasil

3. Fundação Faculdade Federal de Ciências Médicas de Porto Alegre, Brasil

Abstract

Human Immunodeficiency Vírus Type 1 and 2 antibodies detection was performed in 457 dried whole blood spots samples (S&S 903). Q-Preven HIV 1+2 was the screening test used. The results were compared with the gold standard serum tests by ELISA (Cobas Core e Axsym HIV1/2 gO) and imunofluorescence was the definitive confirmatory test. The samples were obtained from the Hospital Nossa Senhora da Conceição in Porto Alegre, RS - Brazil, through whole blood transfer to filter paper card and sent to Caxias do Sul, RS - Brazil where the tests were performed. The dried whole blood spot stability was evaluated with two different panels. The first one was composed of five negative and five positive samples stored at room temperature, 4 ºC, -20 ºC and -70 ºC, while the second was composed of two negative and three positive samples stored at 37 ºC (humidity <50%). Each sample was screened every week for six weeks. These measurement results didn't show variation during the study period. The detected sensibility was 100%, specificity was 99.6%, the positive predictive value was 99.5% and negative predictive values were 100%. The results demonstrated high performance characteristics, opening a new perspective of dried whole blood spot utilization in HIV screening diagnosis.

Publisher

FapUNIFESP (SciELO)

Subject

Infectious Diseases,General Medicine

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