Guided sputum sample collection and culture contamination rates in the diagnosis of pulmonary TB

Author:

Maciel Ethel Leonor Noia1,Prado Thiago Nascimento do2,Peres Renata Lyrio2,Palaci Moises2,Johnson John L.3,Dietze Reynaldo4

Affiliation:

1. Universidade Federal do Espírito Santo, Brazil

2. Federal University of Espírito Santo, Brazil

3. Case Western Reserve University

4. Federal University of Espírito Santo

Abstract

A comparative study to evaluate contamination in cultures of morning sputum samples, comparing those collected at home under currently recommended conditions and those collected under supervision after patient orientation and education. The home and supervised collection groups produced 43 and 76 sputum samples, respectively. The contamination rate was nearly 3-times higher among samples collected at home than among those collected under supervision (37% vs. 13%, p < 0.05; OR = 0.25). The simple educational and hygiene measures described can decrease the contamination rate among sputum samples collected for diagnostic culture.

Publisher

FapUNIFESP (SciELO)

Subject

Pulmonary and Respiratory Medicine

Reference8 articles.

1. The diagnosis of tuberculosis;Brodie D;Clin Chest Med,2005

2. Sputum processing methods to improve the sensitivity of smear microscopy for tuberculosis: a systematic review;Steingart KR;Lancet Infect Dis,2006

3. Better patient instruction for sputum sampling can improve microscopic tuberculosis diagnosis;Alisjahbana B;Int J Tuberc Lung Dis,2005

4. Improvement of tuberculosis case detection and reduction of discrepancies between men and women by simple sputum-submission instructions: a pragmatic randomised controlled trial;Khan MS;Lancet,2007

5. Avaliação do método de Ogawa- Kudoh para o cultivo de micobactérias;Susemihl AA;Rev Bras Pat Clin,1993

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