Affiliation:
1. Claflin University, USA
2. University of Agricultural Sciences and Veterinary Medicine, Romania
Abstract
The objective of this work was to obtain transgenic tomato plants expressing the PfCP-2.9 protein (a chimera of the antigens MSP1 and AMA1 of Plasmodium falciparum). Cotyledons of seven-day-old tomatoes, cultivar Summers, were transformed via Agrobacterium tumefaciens. Transgenic expression in the T0 plants was verified in the DNA extracted from fruits. PCR analysis was used to test the presence of the gene of interest in the T1 generation. Reverse transcriptase PCR provided evidence of gene expression at the RNA level, and Western blot analysis confirmed the presence of the protein of interest in the T1 plants. This is the first report of successful transformation with the expression of a malaria antigen (PfCP-2.9) in transgenic tomato plants from the T0 and T1 generations.
Subject
Agronomy and Crop Science,Animal Science and Zoology
Reference29 articles.
1. Plant-made subunit vaccine against pneumonic and bubonic plague is orally immunogenic in mice;ALVAREZ M.L.;Vaccine,2006
2. Expression of Norwalk virus capsid protein in transgenic tobacco and potato and its oral immunogenicity in mice;ARNTZEN C.J.;Proceedings of the National Academy of Sciences of the United States of America,1996
3. Current protocols in molecular biology;AUSUBEL F.M.,1994
4. Inductions of defense response in tomato against Fusarium Wilt through inorganic chemicals as inducers;BISWAS S.K.;Plant Pathology and Microbiology,2012
5. A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding;BRADFORD M.M.;Analytical Biochemistry,1976
Cited by
9 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献