Affiliation:
1. Universidade Estadual do Norte Fluminense Darcy Ribeiro, Brasil
2. Universidade Estadual do Norte Fluminense Darcy Ribeiro
Abstract
The aim of this work was to evaluate the humus composition from an Ultisol from Campos dos Goytacazes, RJ, Brazil. Soil samples of four depths (0-0.05, 0.05-0.10, 0.10-0.20 and 0.20-0.40 m) and its chemical nature were analysed by elemental composition, E4/E6 ratios and Fourier transformed infrared spectroscopy. The bioactivity of these humified substances was evaluated through their action on maize root growth and H+-ATPase activity of roots microsomes. In topsoil, the content of high condensed alkaline soluble humic substances is greater than that found in the subsuperficial layers. The chemical nature of humic and fulvic acids also varied with the soil depth. The humic acids isolated from the soil samples exhibited higher bioactivity compared with the fulvic acids. Moreover, the results suggest that more condensed humic substances can promote highest stimulation of the microsomal H+-ATPases from maize roots. These data reinforce the concept that the activity of the H+ pumps can be used as a biochemical marker for evaluation of humic substances bioactivity.
Subject
Agronomy and Crop Science,Animal Science and Zoology
Reference28 articles.
1. Agroecology: the science of natural resource management for poor farmers in marginal environments;ALTIERI M.A.;Agriculture,2002
2. Chemical and spectroscopic characterization of humic acids isolated from different Slovak soil types;BARANCIKOVÁ G.;Geoderma,1997
3. Some effects of organic-promotion substances (auximones) on the growth of Lema minor in mineral cultural solutions;BOTTOMLEY W.B.;Proceedings of the Royal Society of London,1917
4. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding;BRADFORD M.;Analytical Biochemistry,1976
5. Organic matter fractions in a toposequence of six soils in the State of Rio de Janeiro, Brazil;CANELLAS L.P.;Pesquisa Agropecuária Brasileira,2000
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