Interception of Wheat mosaic virus (WMoV) in Brazil in maize seeds from the United States-Reference-Cited by-同舟云学术

Interception of Wheat mosaic virus (WMoV) in Brazil in maize seeds from the United States

Published:2016-05 Issue:5 Volume:51 Page:688-691
ISSN:0100-204X
Container-title:Pesquisa Agropecuária Brasileira
language:
Short-container-title:Pesq. agropec. bras.

Author:

Botelho Stephanie Regina Alves¹,Duarte Macária Ferreira²,Barbosa Andreza Viveiros²,Lau Douglas³,Sanches Márcio Martinello⁴,Fernandes Fernanda Rausch⁵

Affiliation:

1. Universidade Católica de Brasília, Brazil

2. Faculdade Anhanguera, Brazil

3. Embrapa Trigo, Brazil

4. Embrapa Recursos Genéticos e Biotecnologia, Brazil

5. Embrapa Quarentena Vegetal, Brazil

Abstract

Abstract: The objective of this work was to evaluate the phytosanitary aspect of two accessions of maize (Zea mays) seeds from the United States introduced to Brazil, regarding the presence of Wheat mosaic virus (WMoV). Two to three weeks after sowing, symptomatic leaves were tested by Elisa using specific antiserum to WMoV. The reaction was positive, and leaf samples were analyzed by real-time PCR and amplified PCR products were sequenced. The WMoV isolates had 99 to 100% nucleotide identity with isolates from Australia and the United States. Until now, there is no report of the presence of this virus in Brazil. According to the federal law on plant protection, the plants were burned to avoid the introduction of this exotic pest in the country. The obtained results show WMoV interception in Brazil.

Publisher

FapUNIFESP (SciELO)

Subject

Agronomy and Crop Science,Animal Science and Zoology

Link

http://www.scielo.br/pdf/pab/v51n5/1678-3921-pab-51-05-00688.pdf

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