New records of Leptospira spp. in wild marsupials and a rodent in the eastern Brazilian Amazon through PCR detection

Author:

SOUZA ROCHA Katarine de1,SCHUPP DE SENA MESQUITA Gleiciane1,SILVA FERREIRA Maeli Fernanda1,LEITE BARROS Flávia de Nazaré1,MACEDO Renata Cecília Soares de Lima1,ARAÚJO SARAIVA Elane de1,MENDES-OLIVEIRA Ana Cristina1,DUARTE CERQUEIRA Valíria1,SCOFIELD Alessandra1,GOES CAVALCANTE Gustavo1,ABEL Isis1,GUIMARÃES DE MORAES Carla Cristina1ORCID

Affiliation:

1. Universidade Federal do Pará (UFPA), Brazil

Abstract

ABSTRACT We analyzed the presence of Leptospira spp. in liver and kidney tissue of wild marsupials and rodents trapped in a periurban forest in the eastern Brazilian Amazon. We examined 25 individuals of four marsupial and seven rodent species for the presence of the 16S rRNA gene of Leptospira in the DNA extracted from 47 liver and kidney tissue samples using PCR. We detected positive samples in 12% (3/25) of the individuals, in kidney fragments of two marsupial species (Didelphis marsupialis and Marmosops pinheiroi) and in a liver fragment of one rodent species (Echimys chrysurus). These are the first records of Leptospira spp. in M. pinheiroi and E. chrysurus and it is the first molecular survey of marsupials and rodents in the Brazilian Amazon.

Publisher

FapUNIFESP (SciELO)

Subject

General Agricultural and Biological Sciences

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