Investigation of toxic factors affecting cells of rat brains exposed to 3-methylcatechol-Reference-Cited by-同舟云学术

Investigation of toxic factors affecting cells of rat brains exposed to 3-methylcatechol

Published:2007-09 Issue:5 Volume:50 Page:839-849
ISSN:1516-8913
Container-title:Brazilian Archives of Biology and Technology
language:
Short-container-title:Braz. arch. biol. technol.

Author:

Barreto George Emílio Sampaio¹,Oliveira Elineusa Silva de²,Villar Flávio Augusto Guerreiro Aragão de²,Santos Gleide Souza dos²,Lima Rute Maria Ferreira²,Costa Silvia Lima²,Costa Maria de Fátima Dias²,Egito Eryvaldo Sócrates Tabosa³,El-Bachá Ramon Santos²

Affiliation:

1. Universidade Federal da Bahia, Brasil; Universidade Federal do Rio Grande do Norte, Brasil

2. Universidade Federal da Bahia, Brasil

3. UFRN, Brasil

Abstract

The aim of this work was to study the effects of 3MC on the peroxidation of biomolecules in nuclear fractions and nonsynaptic mitochondrial respiration in organelles obtained from rat brains. The cytotoxicity towards rat primary astrocytes in vitro was also tested. 3MC at 1mM oxidized consuming oxygen at a rate of 1.98 ± 0.19 µM.min-1 and formed reactive quinones. At the same concentration, 3MC induced peroxidation of biomolecules in nuclear fractions obtained from rat brain homogenates and inhibited state 2 FADH2-linked respiration in nonsynaptic mitochondria. Furthermore, 3MC oxidized in the culture medium, leading to the formation of quinones. This toluene metabolite was cytotoxic to rat primary astrocytes. The concentration that killed 50% of cells after 72 h was 107 mM. The results of the study indicated a direct relationship between cytotoxicity and 3MC oxidation.

Publisher

FapUNIFESP (SciELO)

Subject

Multidisciplinary

Link

http://www.scielo.br/pdf/babt/v50n5/a12v50n5.pdf

Reference37 articles.

1. Organic solvent-induced proximal tubular cell apoptosis via caspase-9 activation;Al-Ghamdi S. S.;Environ. Toxicol. Pharmacol.,2004

2. In vitro response of the numan breast cancer cell line MDAMB-231 and human peripheral blood mononuclear cells exposed to60Co at single fraction;Andrade L. M.;Braz. Arch. Biol. Technol.,2005

3. Oxygenase enzymes and screening method;Arnold F. H.,2005

4. Catechol inhibits FADH2- linked respiration in rat liver mitochondrial fraction;Barreto G. E. S.;Acta Cir. Bras.,2005

5. Learning and memory deficits in rats induced by chronic thinner exposure are reversed by melatonin;Baydas G.;J. Pineal Res.,2005

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