Leaf anatomy and carbon isotope composition in Coffea species related to photosynthetic pathway

Author:

Carelli Maria Luiza Carvalho1,Queiroz-Voltan Rachel Benetti2,Fahl Joel Irineu1,Trivelin Paulo César Ocheuze3

Affiliation:

1. Instituto Agronômico de Campinas, Brasil

2. Instituto Agronômico de Campinas

3. USP

Abstract

Possible presence of vascular bundle sheath cells and its relation to photosynthetic pathway, leaf anatomy and carbon isotope composition (delta13C) were examined in six species of genus Coffea: C. arabica (cvs. Catuaí Vermelho, Mundo Novo, Bourbon Vermelho and Icatu Amarelo), C. canephora (cvs. Apoatã and Guarini), C. liberica, C. dewevrei, C. salvatrix and C. stenophylla. In all genotypes, the vascular bundle was surrounded by a layer of cells with numerous chloroplasts in a centrifugal position. Visually no differences could be seen between the spongy parenchyma cells and the bundle sheath cells, neither in size nor in chloroplast number. The leaf delta13C values ranged between a maximum of - 26.2 ‰ in C. salvatrix and a minimum of -29.7 ‰ in C. liberica. A strong correlation (r = 0.972, p = 0.001) between delta13C and anatomical characteristics was observed in coffee species. C. salvatrix exhibited the highest delta13C values and the most compact mesophyll, with more palisade and spongy parenchyma cells in contact with the vascular bundle sheath.

Publisher

FapUNIFESP (SciELO)

Subject

Plant Science,Agronomy and Crop Science

Reference27 articles.

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2. Structure and distribution of chloroplasts and other organelles in leaves with various rates of photosynthesis;Black Jr CC;Plant Physiol.,1971

3. Coffee: Botany, Biochemistry and production of beans and beverage;Cannel MGR,1987

4. Carbon isotope discrimination and gas exchange in Coffea species grown under different irradiance regimes;Carelli MLC;Rev. Bras. Fisiol. Veg.,1999

5. Caracteristicas anatomicas de las hojas y su relación con el posible ciclo fotosintético en café;Castaño FJO;Cenicafé,1974

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