In vitro establishment of pepper-rosmarin nodal segments

Abstract

Pepper-rosmarin (Lippia sidoides Cham.) is a native medicinal plant from the Brazilian biome Caatinga. Its high economical importance comes from the antimicrobial properties of thymol and carvacrol, both present in the pepper-rosmarin essential oil. Nodal segments of pepper-rosmarin were established in vitro to evaluate different growth regulators. We tested four concentrations of IBA (0.0; 0.01; 0.05; and 0.1 mg L-1) combined with six concentrations of BAP (0.0; 0.1; 0.5; 1.0; 2.0; and 4.0 mg L-1) in assay 1; five concentrations of NAA (0.0; 0.1; 0.25; 0.5; and 1.0 mg L-1) in assay 2; two concentrations of NAA (0.0 and 0.01 mg L-1) combined with five concentrations of kinetin (0.0; 0.25; 0.5; 1.0; and 2.0 mg L-1) in assay 3; five concentrations of IAA (0.0; 0.5; 1.0; 1.5; and 2.0 mg L-1) in assay 4; and five concentrations of GA3 (0.0; 0.1; 0.5; 1.0; and 1.5 mg L-1) in assay 5. The use of BAP, IBA, NAA and KIN did not influence the number of shoots. The addition of 0.1 mg L-1 of NAA at the culture medium resulted in larger shoot length per explant, while the concentration of 1.0 mg L-1 of kinetin promoted larger shoot length in general. GA3 did not affect the elongation of pepper-rosmarin shoots when cultivated in vitro.