Cytotoxicity analysis of electrostatically applied epoxy coating onto CO-CR alloy

Abstract

Objective: To investigate the biological effect of a new method to camouflage the cobalt-chromium (CoCr) metal structure of an RPD, onto which an electrostatic paint was applied. Methods: In vitro cytotoxicity of epoxy Politherm NOBAC30C (Weg Industries SA, Santa Catarina, Brazil) in combination with polished CoCr was tested by placing it in contact with cultured human fibroblasts and comparing it with polystyrene (control surface). The cells were cultured in the presence of the test surfaces for 24, 48, 72, 94 and 120 hours. The number of viable and non-viable cells was established by manual counting. The Tukey test was used to statistically analyze cell counts between the groups. Results: The results showed that cell proliferation was similar between the groups (p =0.2174). It was observed that at 24, 48 and 72 h, there was no significant increase in cell proliferation in all groups. From 96 to 120 h, an increase in cell proliferation was observed in all groups, with no significant difference between them (p>0.05). Conclusion: The epoxy paint studied showed no cytotoxicity in vitro.