Mutagenic activity of airborne particulate matter from the urban area of Porto Alegre, Brazil

Author:

Vargas Vera Maria Ferrão1,Horn Rubem Cesar1,Guidobono Régis Rolim1,Mittelstaedt Ana Beatriz1,Azevedo Irascema Girardi de1

Affiliation:

1. Fundação Estadual de Proteção Ambiental Henrique Luis Roessler (FEPAM)

Abstract

The mutagenic activity of airborne particulate matter collected from three different sites within the urban area of Porto Alegre, Brazil, was investigated using a Salmonella/microsome assay. Samples were extracted by sonication, sequentially, with cyclohexane (CX), and dichloromethane (DCM), for a rough fractionation by polarity. The different fractions were tested for mutagenicity using Salmonella typhimurium strains TA98, with and without metabolic activation (S9 mix fraction), and TA98NR and TA98/1,8-DNP6, without metabolic activation. Mutagenic response was observed for frameshift strain TA98 in assays with and without metabolization for two sites (sites 2 and 3), which had considerable risk of environmental contamination by nonpolar (CX) and/or moderately polar (DCM) compounds. However, the values of revertants/m3 (rev/m3) were highest on the site subject to automobile exhaust (site 3) in assays without (9.56 rev/m3) and with metabolization (5.08 rev/m3). Maximum mutagenic activity was detected in the moderately polar fraction, decreasing after metabolization. Nevertheless, the nonpolar fractions (CX) gave higher mutagenic activity in the presence of metabolization than in the absence of the S9 mix fraction. The responses observed for TA98NR and TA98/1,8-DNP6 strains suggest the activity of nitrocompounds.

Publisher

FapUNIFESP (SciELO)

Subject

Genetics,Molecular Biology

Reference26 articles.

1. Mutagenicity of organic extracts from Santiago (Chile) airborne particulate matter;Adonis M.;Mutat. Res.,1993

2. Material particulado em suspensão no ar ambiente: Determinação de concentração total pelo método do amostrador de grande volume. NBR 9547,1988

3. Mutagenicity of airborne particles from a nonindustrial town in Italy;Barale R.;Environ. Molec. Mutagen.,1989

4. An investigation of interurban variations in the chemical composition and mutagenic activity of airborne particulate organic matter using an integrated chemical class/bioassay system;Butler J.P.;Atmos. Environ.,1987

5. Mutagenicity of airborne particles;Chrisp C.E.;Mutat. Res.,1980

Cited by 25 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3