Genetic diversity characterization of cassava cultivars (Manihot esculenta Crantz).: I) RAPD markers

Author:

Colombo Carlos1,Second Gérard2,Valle Tereza Losada1,Charrier André3

Affiliation:

1. Instituto Agronômico (IAC)

2. ORSTOM

3. ENSAM (Ecole Nationale Supérieure Agronomique de Montpellier)

Abstract

RAPD markers were used to investigate the genetic diversity of 31 Brazilian cassava clones. The results were compared with the genetic diversity revealed by botanical descriptors. Both sets of variates revealed identical relationships among the cultivars. Multivariate analysis of genetic similarities placed genotypes destinated for consumption "in nature" in one group, and cultivars useful for flour production in another. Brazil’s abundance of landraces presents a broad dispersion and is consequently an important resource of genetic variability. The botanical descriptors were not able to differentiate thirteen pairs of cultivars compared two-by-two, while only one was not differentiated by RAPD markers. These results showed the power of RAPD markers over botanical descriptors in studying genetic diversity, identifying duplicates, as well as validating, or improving a core collection. The latter is particularly important in this vegetatively propagated crop.

Publisher

FapUNIFESP (SciELO)

Subject

Genetics,Molecular Biology

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