Molecular analysis of the N gene of canine distemper virus in dogs in Brazil

Author:

Castilho J.G.1,Brandão P.E.1,Carnieli Jr P.1,Oliveira R.N.1,Macedo C.I.1,Peixoto Z.M.P.1,Carrieri M.L.1,Kotait I.1

Affiliation:

1. Instituto Pasteur

Abstract

Eleven central-nervous-system samples collected from stray dogs between 2000 and 2004 were found positive by RT-PCR, which amplified a 480bp fragment of the N gene of canine distemper virus (CDV). Phylogenetic analysis based on partial N-gene sequences showed four major clusters. All dog strains segregated into cluster I, with a mean nucleotide identity of 95.8% and 95.6% with the Onderstepoort and Lederle vaccine strains, respectively. Cluster II contained all the raccoon-related strains, cluster III Orient strains and Cluster IV the Onderstepoort and Lederle vaccine strains, with a mean nucleotide identity of 99.7% between them. This is the first report of phylogenetic analysis of CDV strains in Brazil.

Publisher

FapUNIFESP (SciELO)

Subject

General Veterinary

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