Affiliation:
1. Ministério da Agricultura e do Abastecimento
2. Universidade Federal de Minas Gerais
3. Universidade Federal de Minas Gerais, Brazil
Abstract
An ELISA system was applied to study the prevalence of EHV-1 in vaccinated and non-vaccinated Brazilian horses. Serum samples were taken from non-symptomatic pregnant mares, before and after the parturition, and from their neonatal foals. The antigen was prepared from a purified virus fraction (HVE-1 AB1 strain). The ELISA absorbance of 0.3516 was determined as the threshold of negative values. This cut-off level was defined from a selected interval, which determined a sensibility of 100% and a specificity of 94.7%, from a serum collection obtained from pregnant mares and fetuses. In this study, a strong correlation was found between the ELISA and serum neutralization for specific EHV-1 antibody detection.
Reference22 articles.
1. Molecular epizootiology, pathogenesis and prophylaxis of equine herpesvirus-1 infections;ALLEN G. P.;Prog. Vet. Microbiol. Immunol.,1986
2. Equine infectious disease III;BURROWS R.,1973
3. Epitopes of glycoprotein-G of equine herpesvirus 4 and 1 located near the C-termini elicit type specific antibody responses in the natural host;CRABB B.S;J. Virol.,1993
4. Immunization against viral rhinopneumonitis of horses with lives virus propagated in hamsters;DOLL E.R;J. Am. Vet. Med. Assoc.,1961
5. Incubation periods for abortion in equine viral rhinopneumonitis;DOLL E.R.;J. Am. Vet. Med. Assoc.,1962
Cited by
6 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献