Cloning of the transmembrane glycoproteins G and F from a Brazilian isolate of bovine respiratory syncytial virus in a prokaryotic system-Reference-Cited by-同舟云学术

Cloning of the transmembrane glycoproteins G and F from a Brazilian isolate of bovine respiratory syncytial virus in a prokaryotic system

Published:2011-06 Issue:3 Volume:63 Page:552-558
ISSN:0102-0935
Container-title:Arquivo Brasileiro de Medicina Veterinária e Zootecnia
language:
Short-container-title:Arq. Bras. Med. Vet. Zootec.

Author:

Silva L.H.A.¹,Cardoso K.C.¹,Silva M.J.¹,Spilki F.R.²,Arns C.W.¹

Affiliation:

1. Universidade Estadual de Campinas

2. Universidade Feevale

Abstract

The aim of this work was the cloning of those transmembrane glycoproteins G and F from an isolate bovine respiratory syncytial viruses (BRSV) - a Brazilian isolate of BRSV, named BRSV-25-BR in previous studies, in a prokaryotic system to proceed the sequencing of larger genomic fragments. The nucleotide substitutions were confirmed and these clones may also be used in further studies regarding the biological effects of those proteins in vitro and in vivo.

Publisher

FapUNIFESP (SciELO)

Subject

General Veterinary

Link

http://www.scielo.br/pdf/abmvz/v63n3/v63n3a03.pdf

Reference39 articles.

1. Detection of Brazilian bovine respiratory syncytial virus strain by a reverse transcriptase-nested-polymerase chain reaction in experimentally infected calves;ALMEIDA R.S;Vet. Microbiol,2005

2. Characterization of bovine respiratory syncytial virus isolated in Brazil;ARNS C.W;Braz. J. Med. Biol. Res,2003

3. Immunization with a peptide derived from the G glycoprotein of bovine respiratory syncytial virus (BRSV) reduces the incidence of BRSV associated pneumonia in the natural host;BASTIEN N;Vaccine,1997

4. Chimeric bovine respiratory syncytial virus with glycoprotein gene substitutions from human respiratory syncytial virus (hRSV): effects on host range and evaluation as a live-attenuated hRSV vaccine;BUCHHOLZ U.J;J. Virol,2000

5. Fields virology;COLLINS P.L,2001