Affiliation:
1. UdeLAR, Uruguay
2. Universidad de Zaragoza
Abstract
Fragile sites (FS) are chromosomal regions where the normal compactation of chromatine is not observed. FRAXA (Fra Xq27.3, X sexual chromosome) is one of the most studied FS in humans. FRAXA is an expansion of the trinucleotide CGG located in the gene FMR-1. In cattle, sites of chromosomal fragility were reported in BTAX, associated with different pathologies and fertility impairment. Chromosomal microdissection has became a valuable tool for isolating chromatine fragments. In this work, it was combined the chromosomal microdissection technique with DOP-PCR in order to carry out a molecular analysis of the fragile chromosomal region BTAXq31-34. In that region, polymorphic DNA-RAPD sequences (GC rich) are present and sequences of the gene FMR-1 are missing. The results showed the usefulness of the microdissection-DOP-PCR technique for molecular characterization of fragile chromosomal sites in cattle.
Reference27 articles.
1. Assignment of bovine trophinin (TRO) to the q arm of the X chromosome by fluorescence in situ hybridization;ASAI M.;Anim. Genet.,2004
2. Mutagen-induced chromosome instability in farm animals;DANIELAK-CZECH B.;J. Anim. Feed. Sci.,2004
3. ISCNDB 2000 International System for chromosome nomenclature of domestic bovids;DI BERARDINO D.;Cytogenet. Cell Genet.,2001
4. Generation of chromosome fragment specific bovine ADN sequences by microdissection and DOP-PCR;GOLDAMMER T.;Cytogenet. Cell. Genet.,1996
5. A case of subfertile cow with structural abnormalities of the X chromosome;HANADA H.;Ann. Genet. Sel. Anim.,1980
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