Enzyme linked immunosorbent assay for rubella antibodies: a simple method of antigen production. A preliminary report-Reference-Cited by-同舟云学术

Enzyme linked immunosorbent assay for rubella antibodies: a simple method of antigen production. A preliminary report

Published:1995-08 Issue:4 Volume:37 Page:357-359
ISSN:0036-4665
Container-title:Revista do Instituto de Medicina Tropical de São Paulo
language:
Short-container-title:Rev. Inst. Med. trop. S. Paulo

Author:

Souza Vanda Akico Ueda Fick de¹,Sumita Laura Masami¹,Otsubo Mary Eiko S.²,Takei Kioko¹,Pannuti Cláudio Sérgio¹

Affiliation:

1. Universidade de São Paulo, Brazil

2. Instituto Adolfo Lutz, Brazil

Abstract

A simple method of rubella antigen production by treatment with sodium desoxycholate for use in enzyme immunoassay (IMT-ELISA) is presented. When this assay was compared with a commercial test (Enzygnost-Rubella, Behring), in the study of 108 sera and 118 filter paper blood samples, 96.9% (219/226) overall agreement and correlation coefficient of 0.90 between absorbances were observed. Seven samples showed discordant results, negative by the commercial kit and positive by our test. Four of those 7 samples were available, being 3 positive by HI.

Publisher

FapUNIFESP (SciELO)

Subject

Infectious Diseases,General Medicine

Link

http://www.scielo.br/pdf/rimtsp/v37n4/a13v37n4.pdf

Reference8 articles.

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5. Antigen requirements, sensitivity and specificity of enzyme immunoassays for measles and rubella viral antibodies;FORGHANI B.;J. clin. Microbiol.,1979

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