Gelatin particle indirect agglutination test for serodiagnosis of schistosomiasis: comparative study with enzyme-linked immunosorbent assay

Author:

Kobayashi Jun1,Carrilho Flair José2,Shimabukuro Tetsu3,Silva Luiz Caetano da2,Soares Elza Cotrim3,Nishimura Nancy Fusae3,Chieffi Pedro Paulo4,Sato Yoshiya5

Affiliation:

1. University of Campinas, Brasil; University of the Ryukyus, Japan

2. University of São Paulo, Brasil

3. University of Campinas, Brasil

4. University of São Paulo, Brasil; University of São Paulo, Brasil

5. University of the Ryukyus, Japan; University of the Ryukyus, Japan

Abstract

A new serological test, the gelatin particle agglutination test (GPAT), was used for the serodiagnosis of schistosomiasis mansoni. This technique showed the sensitivity (90.6%) and specificity (97.8%) close to those of enzyme-linked immunosorbent assay. The GPAT can be easily and rapidly performed without specialized equipment, by using lyophilized antigen-coated gelatin particles. The test also seems to be useful for mass screening of Schistosoma infection in field conditions.

Publisher

FapUNIFESP (SciELO)

Subject

Infectious Diseases,General Medicine

Reference31 articles.

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2. Evaluation of enzyme-linked immunosorbent assay (ELISA) as a diagnostic tool for schistosomiasis;BARAKAT R.M.;Trans. roy. Soc. trop. Med. Hyg.,1983

3. Evolution de taux des anticorps fluorescents dans 58 cas de Bilharzioses intestinales ou urinaires traitées par le Nitrothiamidazole;COUDERT J.;Lyon méd.,1968

4. Immunodiagnosis of recently acquired Schistosoma mansoni infection: A comparison of various immunological techniques;DEELDER A.M.;Trop. geogr. Med.,1981

5. The enzyme-linked immunosorbent assay in the diagnosis of human bilharziasis;FARAG H.F.;Tropenmed. Parasit.,1978

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