Detection of mycoplasmas in urethral swabs from HIV-1 infected patients and control individuals using culture techniques and polymerase chain reaction

Author:

CUNHA Regina Ayr Florio da1,TAKEI Kioko1,VAZ Adelaide José1,ROSENTHAL Caio2

Affiliation:

1. Universidade de São Paulo

2. Hospital do Servidor Público Estadual de São Paulo

Abstract

The objective of the present study was to determine the prevalence of certain mycoplasma species, i.e., Mycoplasma hominis, Ureaplasma urealyticum and Mycoplasma penetrans, in urethral swabs from HIV-1 infected patients compared to swabs from a control group. Mycoplasmas were detected by routine culture techniques and by the Polymerase Chain Reaction (PCR) technique, using 16SrRNA generic primers of conserved region and Mycoplasma penetrans specific primers. The positivity rates obtained with the two methods were comparable. Nevertheless, PCR was more sensitive, while the culture techniques allowed the quantification of the isolates. The results showed no significant difference (p < 0.05) in positivity rates between the methods used for mycoplasma detection.

Publisher

FapUNIFESP (SciELO)

Subject

Infectious Diseases,General Medicine

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