Author:
Hamdan Júnia Soares,Resende Maria Aparecida de,Franzot Sarah Piancastelli,Cisalpino Eduardo Osório
Abstract
Yeast forms of five strains of Paracoccidioides brasiliensis (SN, 2, 18, 192 and JT- 1) were cultured in a synthetic medium for obtaining methylic antigens. These antigens were lyophilized and studied for each strain, to determine their partial biochemical composition, through measurements of total lipid, protein and carbohydrate contents. Lipids of methylic antigens were purified and analysed for sterols, phospholipids, glycolipids, li-poproteins, and partial characterization of sterols. Significant differences were found among antigenic preparations derived from distinct P. brasiliensis strains, in relation to the quantitative determinations. On the other hand, sterol analysis revealed the presence of ergosterol, lanosterol and squalene in all samples. The diversity verified in the biochemical characteristics of antigens derived from different P. brasiliensis strains, confirm the need of using a pool of fungal samples in order to produce antigen preparations for serological procedures without hampering their sensitivity.
Subject
Infectious Diseases,General Medicine
Reference35 articles.
1. A comparative study of eight Paracoccidioides brasiliensis isolates;BIAGIONI L.M.V.;Rev. Inst. Med. trop. S. Paulo,1986
2. Spectrophotometric semi-microdetennination of ergosterol in yeast J;BREIVIK O. N.;Agric. Food Chem.,1957
3. Purificación de antígenos somáticos del Paracoccidioides brasiliensis: Estudio preliminar;BURGOS L. C.;Rev. Inst. Med. trop. S. Paulo,1985
4. Production of Paracoccidioides brasiliensis exoantigens for immunodifusion tests;CAMARGO Z. P.;J. din. Microbiol.,1988
5. Predictive value of serologic tests in the diagnosis and follow-up of patients with paracoccidioidomycosis;CANO L. E.;Rev. Inst. Med. trop. S. Paulo,1987
Cited by
2 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献