DOSE RESPONSE EFFECT OF Paracoccidioides brasiliensis IN AN EXPERIMENTAL MODEL OF ARTHRITIS

Author:

Loth Eduardo Alexandre1,Biazim Samia Khalil1,Santos José Henrique Fermino Ferreira dos1,Puccia Rosana2,Brancalhão Rosimeire Costa1,Chasco Lucinéia de Fátima1,Gandra Rinaldo Ferreira1,Simão Rita de Cássia Garcia1,Franco Marcello Fabiano de2

Affiliation:

1. Universidade Estadual do Oeste do Paraná, Brazil

2. Universidade Federal de São Paulo, Brazil

Abstract

Paracoccidioidomycosis (PCM) is caused by the dimorphic fungus Paracoccidioides brasiliensis (Pb) and corresponds to prevalent systemic mycosis in Latin America. The aim of the present work was to evaluate the dose response effect of the fungal yeast phase for the standardization of an experimental model of septic arthritis. The experiments were performed with groups of 14 rats that received doses of 103, 104 or 105 P. brasiliensis (Pb18) cells. The fungi were injected in 50 µL of phosphate-buffered saline (PBS) directly into the knee joints of the animals. The following parameters were analyzed in this work: the formation of swelling in knees infused with yeast cells and the radiological and anatomopathological alterations, besides antibody titer by ELISA. After 15 days of infection, signs of inflammation were evident. At 45 days, some features of damage and necrosis were observed in the articular cartilage. The systemic dissemination of the fungus was observed in 11% of the inoculated animals, and it was concluded that the experimental model is able to mimic articular PCM in humans and that the dose of 105 yeast cells can be used as standard in this model.

Publisher

FapUNIFESP (SciELO)

Subject

Infectious Diseases,General Medicine

Cited by 2 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

1. Osteoarticular Mycoses;Clinical Microbiology Reviews;2022-12-21

2. Natural history of experimental arthritis induced by Paracoccidioides brasiliensis in wistar rats;Revista da Sociedade Brasileira de Medicina Tropical;2019

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