Immunoperoxidase technique using an anti-Leishmania (L.) chagasi hyperimmune serum in the diagnosis of culture-confirmed American tegumentary leishmaniasis-Reference-Cited by-同舟云学术

Immunoperoxidase technique using an anti-Leishmania (L.) chagasi hyperimmune serum in the diagnosis of culture-confirmed American tegumentary leishmaniasis

Published:2009-04 Issue:2 Volume:51 Page:83-86
ISSN:0036-4665
Container-title:Revista do Instituto de Medicina Tropical de São Paulo
language:
Short-container-title:Rev. Inst. Med. trop. S. Paulo

Author:

Quintella Leonardo P.¹,Cuzzi Tullia²,Madeira Maria de F.³,Okamoto Thais³,Schubach Armando de O.⁴

Affiliation:

1. Universidade Federal do Rio de Janeiro; Fundação Oswaldo Cruz, Brazil

2. Universidade Federal do Rio de Janeiro, Brazil

3. Fundação Oswaldo Cruz, Brazil

4. Fundação Oswaldo Cruz, Brazil; Fundação Oswaldo Cruz, Brazil

Abstract

The present study reports the production of the rabbit anti-Leishmania (L.) chagasi hyperimmune serum, the standardization of the immunohistochemistry (IHC) technique and the evaluation of its employment in cutaneous leishmaniasis (CL) lesions diagnosed by Leishmania sp. culture isolation. Thirty fragments of active CL lesions were examined as well as 10 fragments of cutaneous mycosis lesions as control group. IHC proved more sensitive in detecting amastigotes than conventional hematoxylin-eosin (HE) stained slides: the former was positive in 24 (80%) biopsies whereas the latter, in 16 (53%) (p = 0.028). The reaction stained different fungus species causing cutaneous mycosis. Besides, positive reaction was noticed in mononuclear and endothelial cells. Nevertheless, this finding was present in the control group biopsies. It is concluded that IHC showed good sensitivity in detecting amastigotes.

Publisher

FapUNIFESP (SciELO)

Subject

Infectious Diseases,General Medicine

Link

http://www.scielo.br/pdf/rimtsp/v51n2/05.pdf

Reference19 articles.

1. An evaluation of clinical, serologic, anatomopathologic and immunohistochemical findings for fifteen patients with mucosal leishmaniasis before and after treatment;AMATO V.S;Rev. Inst. Med. trop. S. Paulo,1998

2. Mucosal leishmaniasis: in situ characterization of the host inflammatory response, before and after treatment;AMATO V.S;Acta trop.,2003

3. American tegumentary leishmaniasis (ATL) in Rio de Janeiro State, Brazil: main clinical and epidemiologic characteristics;DE OLIVEIRA-NETO M.P;Int. J. Derm.,2000

4. Value of diagnostic techniques for cutaneous leishmaniasis;FABER W.R;J. Amer. Acad. Derm.,2003

5. Tratado de infectologia;FALQUETO A,2005

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