Dot-ELISA for the detection of anti-Cysticercus cellulosae antibodies in cerebrospinal fluid using a new solid phase (resin-treated polyester fabric) and Cysticercus longicollis antigens

Author:

Vaz Adelaide José1,Nakamura Paulo Mutuko2,Camargo Mario E.3,Camargo Eide Dias2,Ferreira A. Walter3

Affiliation:

1. Universidade de São Paulo, Brasil

2. Instituto Adolfo Lutz, Brasil

3. Instituto de Medicina Tropical de São Paulo, Brasil

Abstract

A dot-ELISA was developed for the detection of antibodies in CSF in the immunologic diagnosis of human neurocysticercosis, using antigen extracts of the membrane and scolex of Cysticercus cellulosae (M+S-Cc) and, alternately, membrane (M) and vesicular fluid (VF) of Cysticercus longicollis (Cl) covalently bound to a new solid phase consisting of polyester fabric treated with N-methylol-acrylamide resin (dot-RT). The test was performed at room temperature, with reduced incubation times and with no need for special care in the manipulation of the support. The sensitivity rates obtained were 95.1% for antigen Cc and 97.6% for antigen Cl. Specificity was 90.6% when Cc was used, and 96.9% and 100% when M-Cl and VF-Cl were used, respectively. No significant differences in titer were observed between tests carried out with homologous and heterologous antigens. The low cost and easy execution of the dot-RT test using antigen extracts of Cysticercus longicollis indicate the test for use in the immunodiagnosis of human neurocysticercosis.

Publisher

FapUNIFESP (SciELO)

Subject

Infectious Diseases,General Medicine

Reference21 articles.

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3. Immunodiagnosis of human and porcine cysticercosis. Detection of antibodies and parasite products;CORREA D.;Acta leidensia,1989

4. Studies on the biology of Taenia crassiceps;FREEMAN R.S.;Canad. J, Zool.,1962

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