Simplification of Immune Adherence Hemagglutination test for detection of rabies antibodies in human serum

Author:

Zanetti Carlos Roberto1,Tino Milene Silva2,Chamelet Esther Luiza Bocato1,Ishizuka Masaio Mizuno3,Pereira Octavio Augusto de Carvalho2

Affiliation:

1. Instituto Pasteur de São Paulo, Brazil

2. Escola Paulista de Medicina, Brazil

3. Universidade de São Paulo, Brazil

Abstract

In the present work the immune adherence hemagglutination test (IAHA) was standardized in a simplified procedure. This test showed good reproducibility, better than the classical mice serum neutralization test (SN). The tests showed high correlation degree: high titers in one test corresponded to high titers in the other one, and the same occured with low titers. The IAHA test is extremely simple, fast to perform, and of low cost when compared to tests such as SN or indirect immunofluorescense (IIF). It also proved to be useful in less sophisticated laboratories or even as a screening test for the titration of rabies antibodies.

Publisher

FapUNIFESP (SciELO)

Subject

Infectious Diseases,General Medicine

Reference12 articles.

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2. Rapid test for detection of rabies antibodies in human serum;BUDZKO D. B.;J. clin. Microbiol.,1983

3. Detection of antibody to varicella-zoster virus by immune adherence hemagglutination;GERSHON A.;Proc. Soc. exp. Biol. (N.Y.),1976

4. Fluorescent antibody staining of street and fixed rabies virus antigens;GOLDWASSER R. A.;Proc. Soc. exp. Biol. (N.Y.),1958

5. Produção da vacina anti-rábica veterinária em suspensão de células BHK;GUIDOLIN R.;Rev. Microbiol. (S. Paulo),1983

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2. Immunomodulatory effect of glucan on the response to experimental antirabies vaccination;Revista do Instituto de Medicina Tropical de São Paulo;1993-10

3. Simplified fluorescent inhibition microtest for the titration of rabies neutralizing antibodies;Revista do Instituto de Medicina Tropical de São Paulo;1993-04

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