Evaluation of enzyme-linked immunosorbent and alternative assays for detection of HIV antibodies using panels of Brasilian sera

Author:

Ivo-Dos-Santos Jairo1,Mello Deise L. Campos1,Couto-Fernandez José C.1,Passos Roberto M.1,Dias-Carneiro Leila A.1,Castilho Euclides A.1,Galvão-Castro Bernardo1

Affiliation:

1. Fundação Oswaldo Cruz, Brasil

Abstract

Sera from 472 Brazilian subjects, confirmed to be either positive or negative for HIV antibodies and comprising the total clinical spectrum of HIV infection, were utilized in the evaluation of six commercially available enzyme-linked immunosorbent assays (ELISA), as well as of four alternative assays, namely indirect immunofluorescence (IIF), passive hemagglutination (PHA), dot blot and Karpas AIDS cell test. The sensitivities ranged from 100% (Abbott and Roche ELISA) to 84.2% (PHA) and the specificities ranged from 99.3% (IIF) to 80.2% (PHA). The sensitivity and specificity of the PHA and the sensitivity of the Karpas cell test were significantly lower than those of the other tests. Although the IFF and dot blot had good sensitivities and specificities, the six ELISA were more attractive than those tests when other parameters such as ease of reading and duration of assay were considered.

Publisher

FapUNIFESP (SciELO)

Subject

Infectious Diseases,General Medicine

Reference14 articles.

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