Polyvinyl alcohol-glutaraldehyde as solid-phase in Elisa for Schistosomiasis-Reference-Cited by-同舟云学术

Polyvinyl alcohol-glutaraldehyde as solid-phase in Elisa for Schistosomiasis

Published:1997-05 Issue:3 Volume:39 Page:155-158
ISSN:0036-4665
Container-title:Revista do Instituto de Medicina Tropical de São Paulo
language:
Short-container-title:Rev. Inst. Med. trop. S. Paulo

Author:

ARAÚjO Aureci M.¹,BARBOSA Gustavo H.T.S.²,DINIZ José Ricardo P.²,MALAGUEÑO Elizabeth³,AZEVEDO Walter M.⁴,CARVALHO Jr. Luiz B. de³

Affiliation:

1. Universidade Federal de Pernambuco (UFPE); HEMOPE - Fundação de Hematologia e Hemoterapia de Pernambuco

2. Universidade Federal de Pernambuco (UFPE)

3. Universidade Federal de Pernambuco (UFPE); UFPE

4. UFPE

Abstract

Soluble adult Schistosoma mansoni antigen preparation (SWAP) was covalently fixed onto polyvinyl alcohol-glutaraldehyde discs and an enzyme linked-immunosorbent assay (ELISA) was set up. The best conditions for the assay were established and it was found that small amount of antigen such as 1.5 µg was required. A comparison between this procedure and the conventional ELISA was proceeded. A reliable method of antigen immobilization was achieved and the low prices of the employed reagents are economically attractive

Publisher

FapUNIFESP (SciELO)

Subject

Infectious Diseases,General Medicine

Link

http://www.scielo.br/pdf/rimtsp/v39n3/0029.pdf

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