Author:
Aliniya Nora,Pouresmaeili Farkhondeh,Seifi Alan Mahnaz,Ghafourifard Soudeh
Abstract
For research on gene expression and its changes, maintenance of solid tissues like placenta is very important. Single–stranded RNA fragility under different environmental effects has prompted researchers to develop new RNA storage protocols to reduce entered damage to the tissue until extraction time. The applications of liquid nitrogen and RNA latter have been commonly used by investigators. We were looking for the simplest way to keep placental samples in appropriate condition with great RNA stability and function. Our experiments showed that storage of placental specimens in conventional freezing condition at –15 to –20 for short time and transfer at – 80 for longer time is as respondent as when applying liquid nitrogen or RNA later reagent.