Experimental study: a comparison of the uro-protective efficacy of mesna with hyperhydration in cyclophosphamide-induced haemorrhagic cystitis in sprague dawley rats

Abstract

Background: Haemorrhagic Cystitis (H.C) is well known as a dose-limiting side effect of cyclophosphamide (CyP). The aim of this study was to investigate the effect of Mesna in protecting against CyP-induced H.C and compare its efficacy to that of hyperhydration. Research design: This animal study was conducted at the Hospital Clinic de Barcelona's Experimental Animals Breeding and Medical Research Centre. Materials and Methodology: Male Sprague Dawley rats (360-510 g, 5 rats per group) were divided into four groups at random: No medications were given to Group 1 (Control group). Group 11 (received CyP 150mg/kg intraperitoneally alone), Group 111 (received CyP150mg/kg, and mesna 150 percent of the CyP dose immediately and at 4 and 8 hours after administration of CyP, both drugs IV injection by intra jugular vein), and Group 1V (received CyP150mg/kg, and hyperhydration by catheterization of the animal jugular vein with a constant infusion of glucose water 5%, 3ml/hour/48 hours). 48 hours later, the bladders of the animals were examined macroscopically and histologically. The existence of edema and haemorrhagic edema, as well as histological and gross evaluations of bladder injury, were assessed, and statistical analysis was employed. Results: HC was found in all of the animals in group 11. When compared to group 1 (p0.001), this group had significantly more histological damage and macroscopic alterations. When compared to group 11, the median ratings of bladder injury in groups 111 and 1V were considerably lower. There was significant difference between groups 11 apart, and both groups111, 1V when the median scores for bladder injury were compared. Conclusion: This study shows the efficacy of mesna or hyperhydration in the prevention of cyclophosphamide-induced haemorrhagic cystitis.