Abstract
The study of the content of biologically active substances in the samples of the bark-wood waste destruction products (BWW) was continued. BWW were taken at a depth of 1 to 27 meters of the bark dump of OJSC Syktyvkar LDK, located in the Forestry microdistrict of Syktyvkar. The hexane-soluble substances content is in ranges from 0.78 to 1.89% of the dry mass of BWW. The minimum content was found in the first (0.24%) and last samples (0.21%) at the minimum and maximum depth, the maximum – in samples at a depth of 3 to 9 m (1.66–1.89%). The variation of the hexane extract (HE) content in BWW’s dry mass depending on the depth of occurrence is not clear. The HE content in the dry ethyl acetate extract is mainly within 50%. The minimum content was found in the first sample at a depth of up to 1.5 m (34.9%), the maximum in the last sample at a depth of 27.0 m, where it reaches 66% of the dry weight of the ethyl acetate extract. The analysis of the obtained hexane extracts by thin-layer chromatography showed that the HE contains substances with a mobility coefficient of 0.61, which coincide with dihydroquercetin and some phenolic acids previously detected in ethyl acetate extracts. Six phenolic compounds were detected by high-performance liquid chromatography in hexane extracts. Protocatechic acid, vanillic acid, trans-p-coumaric acid and its cis isomer were identified by chromatographic and spectral characteristics.